normal human liver epithelial cell line  (ATCC)

Journal: BMC Cancer

Article Title: Circ_0064288 acts as an oncogene of hepatocellular carcinoma cells by inhibiting miR-335-5p expression and promoting ROCK1 expression

doi: 10.1186/s12885-022-09323-8

Figure Lengend Snippet: Circ_0064288 expression is up-modulated in HCC. A - B . Volcano plots were utilized to show the differentially expressed circRNAs in HCC, with the data of GSE94508 and GSE97332. C . Venn diagram was employed to screen the circRNAs that were remarkably up-modulated in both datasets. D . Circ_0064288 expression in HCC tissues and paracancerous tissues was examined by qRT-PCR, and then the fold change of circ_0064288 expression in each HCC sample was shown. E . Kaplan-Meier curve was applied to analyze the relationship between circ_0064288 expression and the overall survival of HCC patients. F . Circ_0064288 expression in normal hepatic epithelial cell line (THLE-3) and HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L) was measured by qRT-PCR. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Human HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L) and human normal liver epithelial cell line (THLE-3) were procured from American Type Culture Collection (ATCC, Manassas, VA, USA) and Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Cancer

Article Title: Circ_0064288 acts as an oncogene of hepatocellular carcinoma cells by inhibiting miR-335-5p expression and promoting ROCK1 expression

doi: 10.1186/s12885-022-09323-8

Figure Lengend Snippet: Circ_0064288 functions as ceRNA and sponges miR-335-5p. A . Bioinformatics databases (StarBase and Circular RNA Interactome) were used to predict the binding site of circ_0064288 to miR-335-5p. B . qRT-PCR was employed to detect circ_0064288 expression in the cytoplasm and nucleus of Huh7 and Hep3B cells. C . Dual-luciferase reporter gene experiment showed that the predicted binding sequence between circ_0064288 and miR-335-5p was functional. D . qRT-PCR was employed to detect miR-335-5p expression in HCC tissues and paracancerous tissues. E . qRT-PCR was used to detect miR-335-5p expression in normal hepatic epithelial cell line (THLE-3) and HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L). F . The correlation between circ_0064288 expression and miR-335-5p expression in HCC tissues was evaluated by Pearson’s correlation coefficient. *** P < 0.001, ns, means no statistical significance. *** P < 0.001, ns, means no statistical significance

Article Snippet: Human HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L) and human normal liver epithelial cell line (THLE-3) were procured from American Type Culture Collection (ATCC, Manassas, VA, USA) and Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China).

Techniques: Binding Assay, Quantitative RT-PCR, Expressing, Luciferase, Sequencing, Functional Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Integrin α7 knockdown suppresses cell proliferation, migration, invasion and EMT in hepatocellular carcinoma

doi: 10.3892/etm.2021.9740

Figure Lengend Snippet: ITGA7 expression levels in cell lines. (A) ITGA7 mRNA expression in HCC cell lines and human normal liver epithelial cell line. (B and C) ITGA7 protein expression in HCC cell lines and human normal liver epithelial cell line. ITGA7, integrin α7; HCC, hepatocellular carcinoma. * P<0.05, ** P<0.01 and *** P<0.001 vs. THLE-3 cells.

Article Snippet: Human normal liver epithelial cells (THLE-3) and HCC cell lines SKHEP1 and SNU449 were purchased from American Type Culture Collection.

Techniques: Expressing